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An overview of some enzymes from buthid scorpion venoms from Colombia: Centruroides margaritatus, Tityus pachyurus, and Tityus n. sp. aff. metuendus

Leydy Lorena Mendoza-Tobar1, Herlinda Clement2, Iván Arenas2, Juan Carlos Sepulveda-Arias3 ,  Jimmy Alexander Guerrero Vargas1, Gerardo Corzo2* [ + show more ]

J Venom Anim Toxins incl Trop Dis, 2024, 30:e20230063
Received: 12 September 2023 | Accepted: 04 December 2023 | Published online: 18 March 2024


Background: In Colombia, several species of Buthidae scorpions belonging to the genera Centruroides and Tityus coexist, and their stings are considered life-threatening to humans because of their venom neurotoxins. Despite previous studies focusing on neurotoxins from these scorpion genera, little is known about the enzymes present in their venoms and their relationship with whole venom toxicity. Methods: Here, using proteomic and biochemical protocols the enzymatic activities of the venoms of three Colombian scorpion species, C. margaritatus, T. pachyurus, and T. n. sp. aff. metuendus, were compared to establish the presence and absence of enzymes such as phospholipases, hyaluronidases, and proteases that could be related to venom toxicity. Results: C. margaritatus was positive for hyaluronidases, T. n. sp. aff. metuendus for proteases, and T. pachyurus exhibited activity for all three mentioned enzymes. Conclusion: This information provides valuable insights into the specific enzyme diversity of each species’ venom and their potential role in venom toxicity, which could contribute to the development of better treatments and prevention strategies for scorpion envenomation.


Keywords: Scorpion venom; Enzymes; Tityus; Centruroides; Phospholipase

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